The warning message "No instrument results available" is set when the target calling in your Assay Plugin is set to use the PCR Instrument calling instead of the Artificial intelligence.
For Assay Plugins created in FastFinder Studio from version 1.2.2 onwards, a warning message "No instrument results available." is added when FastFinder could not retrieve the cycler callings and the artificial intelligence calling method is used.
Assay Plugins created in FastFinder Studio before 1.2.2 did not receive this message and the artificial intelligence calling is used to display the results. If you want to add this warning, republish your assay in FastFinder Studio. And activate this new plugin version in FastFinder Analysis via Lab configuration - add new assay. Check How to add an assay? for more information.
Why was the instrument calling not retrieved?
LightCycler
All channels (filter combinations) configured within the Assay Plugin need to be analyzed within the LightCycler software.
Repeat the 'Calculate' step in all three analyses for each filter combination.